Part:BBa_K1598002:Design

Lac induced expression cassette + TPH1 (tryptophan hydroxylase 1)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 126
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

- Lac induced expression cassette (BBa_K314103) was transformed from 2014 iGEM distribution
- TPH1 human gene was codon optimized for E.coli, synthesized and submitted as BBa_K1598001
- parts were digested with S&P and X&P respectively, run on agarose gel, extracted, purified and ligated together

Source

DNA synthesis